A cell culture bottle is a container used for storing and growing cells in cell culture. They are usually made of sterile plastic or glass and have special shapes and designs to maximize cell growth and division.
The shape of a cell culture bottle is usually cylindrical, with a slightly rounded bottom to allow cells to be evenly distributed and grow in the bottle. Bottle caps are usually made of sterile plastic and can be easily loosened and closed to facilitate medium replacement without affecting cell growth.
When using cell culture bottles, strict aseptic procedures must be followed to prevent cell contamination. This includes the use of sterile culture media, culture bottles, and operating instruments, as well as operating in a sterile environment when necessary. In addition, it is important to ensure regular replacement of the culture medium and the replacement of the cell culture bottle before the cells reach their maximum density to avoid overcrowding and cell death.
Here are some common specifications for cell culture bottles:
25 milliliter cell culture bottle: usually used for small-scale cultivation, maintenance, and expansion of cells. This type of cell culture bottle typically has a diameter of approximately 22 millimeters, a height of approximately 70 millimeters, and a bottle neck diameter of approximately 14 millimeters.
75ml cell culture bottle: usually used for medium scale cell culture. This type of cell culture bottle typically has a diameter of about 30 millimeters, a height of about 95 millimeters, and a bottle neck diameter of about 20 millimeters.
150ml cell culture bottle: usually used for large-scale culture and expansion of cells. This type of cell culture bottle typically has a diameter of approximately 41 millimeters, a height of approximately 117 millimeters, and a bottle neck diameter of approximately 25 millimeters.
250 milliliter cell culture bottle: usually used for larger scale cell culture and production. This type of cell culture bottle typically has a diameter of approximately 50 millimeters, a height of approximately 137 millimeters, and a mouth diameter of approximately 30 millimeters.
500ml cell culture bottle: usually used to produce more cells and cell cultures. This type of cell culture bottle typically has a diameter of approximately 70 millimeters, a height of approximately 175 millimeters, and a bottle neck diameter of approximately 38 millimeters.
It should be noted that the specifications of cell culture bottles may vary from manufacturer to manufacturer, so when selecting a cell culture bottle, please carefully review the specifications and instructions for use.